cd3positive enrichment kit Search Results


cd3 enrichment assay kit  (Miltenyi Biotec)
99
Miltenyi Biotec cd3 enrichment assay kit
Cd3 Enrichment Assay Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd3positive enrichment kit  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc cd3positive enrichment kit
Cd3positive Enrichment Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
cd3positive enrichment kit - by Bioz Stars, 2026-03
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mouse cd3 t-cell enrichment kit  (STEMCELL Technologies Inc)
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STEMCELL Technologies Inc mouse cd3 t-cell enrichment kit
a The percentages of Tfh (CD4 + CXCR5 + PD-1 + ) and RBD-specific GCB (B220 + GL7 + CD95 + RBD + ) cells in the CLN were analyzed with FCM at 28 days after the last immunization. b , c Lung CD8 + and CD4 + T cells were evaluated for IFN-γ generation by FCM upon restimulation with RBD peptide pools or irrelevant antigens. b Representative FCM plots (left) and quantification (right) of IFN-γ-expressing lung CD8 + T cells. d The proportion of antigen-experienced (CD44 + ) CD4 + and CD8 + T cells expressing CD69 and CD103 in lung tissues. e The percentage of CD103 + DCs coexpressing CD86 and MHC II in the lungs. f Upper left: t-SNE maps were created by concentrating <t>CD3</t> + T cells from the BAL fluid of the vaccinated mice. Analysis was carried out with default FlowJo V.10 software. Upper right and bottom: heatmap projections of CD4, CD8, CD44, CD69, and CD103 expression on t-SNE maps from three independent mice ( n = 3). Red and black hashed circles are indicators of antigen-experienced CD4 + and CD8 + T RM cells in the BAL fluid, respectively. g Absolute numbers of CD8 + T cells producing IFN-γ (left) and TNF-α (right) in the BAL after ex vivo restimulation with RBD peptide pools or irrelevant antigen at 4 weeks after the last immunization. h Quantification of the percentages (left) and the numbers (right) of MHC II + AM in the BAL fluid. Data were displayed with floating bars in ( a – e , g , and h ). The middle line indicates the median and the box shows the data range. Data were presented as mean values ± SEM. n = 4–6 mice in each group in ( a – e , g , and h ). P values in a , d , e , h were calculated with One-way ANOVA followed by Dunnett’s multiple comparisons tests. P values in b , c , and g were calculated with two-way ANOVA followed by Tukey’s multiple comparisons tests. Source data are provided as a Source Data file.
Mouse Cd3 T Cell Enrichment Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse cd3 t-cell enrichment kit/product/STEMCELL Technologies Inc
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mouse cd3 t-cell enrichment kit - by Bioz Stars, 2026-03
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cd3 positive selection kit ii  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc cd3 positive selection kit ii
a The percentages of Tfh (CD4 + CXCR5 + PD-1 + ) and RBD-specific GCB (B220 + GL7 + CD95 + RBD + ) cells in the CLN were analyzed with FCM at 28 days after the last immunization. b , c Lung CD8 + and CD4 + T cells were evaluated for IFN-γ generation by FCM upon restimulation with RBD peptide pools or irrelevant antigens. b Representative FCM plots (left) and quantification (right) of IFN-γ-expressing lung CD8 + T cells. d The proportion of antigen-experienced (CD44 + ) CD4 + and CD8 + T cells expressing CD69 and CD103 in lung tissues. e The percentage of CD103 + DCs coexpressing CD86 and MHC II in the lungs. f Upper left: t-SNE maps were created by concentrating <t>CD3</t> + T cells from the BAL fluid of the vaccinated mice. Analysis was carried out with default FlowJo V.10 software. Upper right and bottom: heatmap projections of CD4, CD8, CD44, CD69, and CD103 expression on t-SNE maps from three independent mice ( n = 3). Red and black hashed circles are indicators of antigen-experienced CD4 + and CD8 + T RM cells in the BAL fluid, respectively. g Absolute numbers of CD8 + T cells producing IFN-γ (left) and TNF-α (right) in the BAL after ex vivo restimulation with RBD peptide pools or irrelevant antigen at 4 weeks after the last immunization. h Quantification of the percentages (left) and the numbers (right) of MHC II + AM in the BAL fluid. Data were displayed with floating bars in ( a – e , g , and h ). The middle line indicates the median and the box shows the data range. Data were presented as mean values ± SEM. n = 4–6 mice in each group in ( a – e , g , and h ). P values in a , d , e , h were calculated with One-way ANOVA followed by Dunnett’s multiple comparisons tests. P values in b , c , and g were calculated with two-way ANOVA followed by Tukey’s multiple comparisons tests. Source data are provided as a Source Data file.
Cd3 Positive Selection Kit Ii, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd3 positive selection kit ii - by Bioz Stars, 2026-03
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cd3 human t-cell enrichment kit  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc cd3 human t-cell enrichment kit
a The percentages of Tfh (CD4 + CXCR5 + PD-1 + ) and RBD-specific GCB (B220 + GL7 + CD95 + RBD + ) cells in the CLN were analyzed with FCM at 28 days after the last immunization. b , c Lung CD8 + and CD4 + T cells were evaluated for IFN-γ generation by FCM upon restimulation with RBD peptide pools or irrelevant antigens. b Representative FCM plots (left) and quantification (right) of IFN-γ-expressing lung CD8 + T cells. d The proportion of antigen-experienced (CD44 + ) CD4 + and CD8 + T cells expressing CD69 and CD103 in lung tissues. e The percentage of CD103 + DCs coexpressing CD86 and MHC II in the lungs. f Upper left: t-SNE maps were created by concentrating <t>CD3</t> + T cells from the BAL fluid of the vaccinated mice. Analysis was carried out with default FlowJo V.10 software. Upper right and bottom: heatmap projections of CD4, CD8, CD44, CD69, and CD103 expression on t-SNE maps from three independent mice ( n = 3). Red and black hashed circles are indicators of antigen-experienced CD4 + and CD8 + T RM cells in the BAL fluid, respectively. g Absolute numbers of CD8 + T cells producing IFN-γ (left) and TNF-α (right) in the BAL after ex vivo restimulation with RBD peptide pools or irrelevant antigen at 4 weeks after the last immunization. h Quantification of the percentages (left) and the numbers (right) of MHC II + AM in the BAL fluid. Data were displayed with floating bars in ( a – e , g , and h ). The middle line indicates the median and the box shows the data range. Data were presented as mean values ± SEM. n = 4–6 mice in each group in ( a – e , g , and h ). P values in a , d , e , h were calculated with One-way ANOVA followed by Dunnett’s multiple comparisons tests. P values in b , c , and g were calculated with two-way ANOVA followed by Tukey’s multiple comparisons tests. Source data are provided as a Source Data file.
Cd3 Human T Cell Enrichment Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd3 human t-cell enrichment kit - by Bioz Stars, 2026-03
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cd3 + t cell enrichment kit easysep human t cell enrichment  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc cd3 + t cell enrichment kit easysep human t cell enrichment
a The percentages of Tfh (CD4 + CXCR5 + PD-1 + ) and RBD-specific GCB (B220 + GL7 + CD95 + RBD + ) cells in the CLN were analyzed with FCM at 28 days after the last immunization. b , c Lung CD8 + and CD4 + T cells were evaluated for IFN-γ generation by FCM upon restimulation with RBD peptide pools or irrelevant antigens. b Representative FCM plots (left) and quantification (right) of IFN-γ-expressing lung CD8 + T cells. d The proportion of antigen-experienced (CD44 + ) CD4 + and CD8 + T cells expressing CD69 and CD103 in lung tissues. e The percentage of CD103 + DCs coexpressing CD86 and MHC II in the lungs. f Upper left: t-SNE maps were created by concentrating <t>CD3</t> + T cells from the BAL fluid of the vaccinated mice. Analysis was carried out with default FlowJo V.10 software. Upper right and bottom: heatmap projections of CD4, CD8, CD44, CD69, and CD103 expression on t-SNE maps from three independent mice ( n = 3). Red and black hashed circles are indicators of antigen-experienced CD4 + and CD8 + T RM cells in the BAL fluid, respectively. g Absolute numbers of CD8 + T cells producing IFN-γ (left) and TNF-α (right) in the BAL after ex vivo restimulation with RBD peptide pools or irrelevant antigen at 4 weeks after the last immunization. h Quantification of the percentages (left) and the numbers (right) of MHC II + AM in the BAL fluid. Data were displayed with floating bars in ( a – e , g , and h ). The middle line indicates the median and the box shows the data range. Data were presented as mean values ± SEM. n = 4–6 mice in each group in ( a – e , g , and h ). P values in a , d , e , h were calculated with One-way ANOVA followed by Dunnett’s multiple comparisons tests. P values in b , c , and g were calculated with two-way ANOVA followed by Tukey’s multiple comparisons tests. Source data are provided as a Source Data file.
Cd3 + T Cell Enrichment Kit Easysep Human T Cell Enrichment, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd3 cd28 activation beads  (Miltenyi Biotec)
99
Miltenyi Biotec cd3 cd28 activation beads
a The percentages of Tfh (CD4 + CXCR5 + PD-1 + ) and RBD-specific GCB (B220 + GL7 + CD95 + RBD + ) cells in the CLN were analyzed with FCM at 28 days after the last immunization. b , c Lung CD8 + and CD4 + T cells were evaluated for IFN-γ generation by FCM upon restimulation with RBD peptide pools or irrelevant antigens. b Representative FCM plots (left) and quantification (right) of IFN-γ-expressing lung CD8 + T cells. d The proportion of antigen-experienced (CD44 + ) CD4 + and CD8 + T cells expressing CD69 and CD103 in lung tissues. e The percentage of CD103 + DCs coexpressing CD86 and MHC II in the lungs. f Upper left: t-SNE maps were created by concentrating <t>CD3</t> + T cells from the BAL fluid of the vaccinated mice. Analysis was carried out with default FlowJo V.10 software. Upper right and bottom: heatmap projections of CD4, CD8, CD44, CD69, and CD103 expression on t-SNE maps from three independent mice ( n = 3). Red and black hashed circles are indicators of antigen-experienced CD4 + and CD8 + T RM cells in the BAL fluid, respectively. g Absolute numbers of CD8 + T cells producing IFN-γ (left) and TNF-α (right) in the BAL after ex vivo restimulation with RBD peptide pools or irrelevant antigen at 4 weeks after the last immunization. h Quantification of the percentages (left) and the numbers (right) of MHC II + AM in the BAL fluid. Data were displayed with floating bars in ( a – e , g , and h ). The middle line indicates the median and the box shows the data range. Data were presented as mean values ± SEM. n = 4–6 mice in each group in ( a – e , g , and h ). P values in a , d , e , h were calculated with One-way ANOVA followed by Dunnett’s multiple comparisons tests. P values in b , c , and g were calculated with two-way ANOVA followed by Tukey’s multiple comparisons tests. Source data are provided as a Source Data file.
Cd3 Cd28 Activation Beads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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positive cd3 cell selection kit ii  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc positive cd3 cell selection kit ii
a The percentages of Tfh (CD4 + CXCR5 + PD-1 + ) and RBD-specific GCB (B220 + GL7 + CD95 + RBD + ) cells in the CLN were analyzed with FCM at 28 days after the last immunization. b , c Lung CD8 + and CD4 + T cells were evaluated for IFN-γ generation by FCM upon restimulation with RBD peptide pools or irrelevant antigens. b Representative FCM plots (left) and quantification (right) of IFN-γ-expressing lung CD8 + T cells. d The proportion of antigen-experienced (CD44 + ) CD4 + and CD8 + T cells expressing CD69 and CD103 in lung tissues. e The percentage of CD103 + DCs coexpressing CD86 and MHC II in the lungs. f Upper left: t-SNE maps were created by concentrating <t>CD3</t> + T cells from the BAL fluid of the vaccinated mice. Analysis was carried out with default FlowJo V.10 software. Upper right and bottom: heatmap projections of CD4, CD8, CD44, CD69, and CD103 expression on t-SNE maps from three independent mice ( n = 3). Red and black hashed circles are indicators of antigen-experienced CD4 + and CD8 + T RM cells in the BAL fluid, respectively. g Absolute numbers of CD8 + T cells producing IFN-γ (left) and TNF-α (right) in the BAL after ex vivo restimulation with RBD peptide pools or irrelevant antigen at 4 weeks after the last immunization. h Quantification of the percentages (left) and the numbers (right) of MHC II + AM in the BAL fluid. Data were displayed with floating bars in ( a – e , g , and h ). The middle line indicates the median and the box shows the data range. Data were presented as mean values ± SEM. n = 4–6 mice in each group in ( a – e , g , and h ). P values in a , d , e , h were calculated with One-way ANOVA followed by Dunnett’s multiple comparisons tests. P values in b , c , and g were calculated with two-way ANOVA followed by Tukey’s multiple comparisons tests. Source data are provided as a Source Data file.
Positive Cd3 Cell Selection Kit Ii, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lin conjugated immunomagnetic negative selection kit  (Miltenyi Biotec)
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Miltenyi Biotec lin conjugated immunomagnetic negative selection kit
a The percentages of Tfh (CD4 + CXCR5 + PD-1 + ) and RBD-specific GCB (B220 + GL7 + CD95 + RBD + ) cells in the CLN were analyzed with FCM at 28 days after the last immunization. b , c Lung CD8 + and CD4 + T cells were evaluated for IFN-γ generation by FCM upon restimulation with RBD peptide pools or irrelevant antigens. b Representative FCM plots (left) and quantification (right) of IFN-γ-expressing lung CD8 + T cells. d The proportion of antigen-experienced (CD44 + ) CD4 + and CD8 + T cells expressing CD69 and CD103 in lung tissues. e The percentage of CD103 + DCs coexpressing CD86 and MHC II in the lungs. f Upper left: t-SNE maps were created by concentrating <t>CD3</t> + T cells from the BAL fluid of the vaccinated mice. Analysis was carried out with default FlowJo V.10 software. Upper right and bottom: heatmap projections of CD4, CD8, CD44, CD69, and CD103 expression on t-SNE maps from three independent mice ( n = 3). Red and black hashed circles are indicators of antigen-experienced CD4 + and CD8 + T RM cells in the BAL fluid, respectively. g Absolute numbers of CD8 + T cells producing IFN-γ (left) and TNF-α (right) in the BAL after ex vivo restimulation with RBD peptide pools or irrelevant antigen at 4 weeks after the last immunization. h Quantification of the percentages (left) and the numbers (right) of MHC II + AM in the BAL fluid. Data were displayed with floating bars in ( a – e , g , and h ). The middle line indicates the median and the box shows the data range. Data were presented as mean values ± SEM. n = 4–6 mice in each group in ( a – e , g , and h ). P values in a , d , e , h were calculated with One-way ANOVA followed by Dunnett’s multiple comparisons tests. P values in b , c , and g were calculated with two-way ANOVA followed by Tukey’s multiple comparisons tests. Source data are provided as a Source Data file.
Lin Conjugated Immunomagnetic Negative Selection Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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spinsep mouse cd3 + t cell enrichment kit  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc spinsep mouse cd3 + t cell enrichment kit
a The percentages of Tfh (CD4 + CXCR5 + PD-1 + ) and RBD-specific GCB (B220 + GL7 + CD95 + RBD + ) cells in the CLN were analyzed with FCM at 28 days after the last immunization. b , c Lung CD8 + and CD4 + T cells were evaluated for IFN-γ generation by FCM upon restimulation with RBD peptide pools or irrelevant antigens. b Representative FCM plots (left) and quantification (right) of IFN-γ-expressing lung CD8 + T cells. d The proportion of antigen-experienced (CD44 + ) CD4 + and CD8 + T cells expressing CD69 and CD103 in lung tissues. e The percentage of CD103 + DCs coexpressing CD86 and MHC II in the lungs. f Upper left: t-SNE maps were created by concentrating <t>CD3</t> + T cells from the BAL fluid of the vaccinated mice. Analysis was carried out with default FlowJo V.10 software. Upper right and bottom: heatmap projections of CD4, CD8, CD44, CD69, and CD103 expression on t-SNE maps from three independent mice ( n = 3). Red and black hashed circles are indicators of antigen-experienced CD4 + and CD8 + T RM cells in the BAL fluid, respectively. g Absolute numbers of CD8 + T cells producing IFN-γ (left) and TNF-α (right) in the BAL after ex vivo restimulation with RBD peptide pools or irrelevant antigen at 4 weeks after the last immunization. h Quantification of the percentages (left) and the numbers (right) of MHC II + AM in the BAL fluid. Data were displayed with floating bars in ( a – e , g , and h ). The middle line indicates the median and the box shows the data range. Data were presented as mean values ± SEM. n = 4–6 mice in each group in ( a – e , g , and h ). P values in a , d , e , h were calculated with One-way ANOVA followed by Dunnett’s multiple comparisons tests. P values in b , c , and g were calculated with two-way ANOVA followed by Tukey’s multiple comparisons tests. Source data are provided as a Source Data file.
Spinsep Mouse Cd3 + T Cell Enrichment Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse cd3 t cell column enrichment kit  (R&D Systems)
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R&D Systems mouse cd3 t cell column enrichment kit
a The percentages of Tfh (CD4 + CXCR5 + PD-1 + ) and RBD-specific GCB (B220 + GL7 + CD95 + RBD + ) cells in the CLN were analyzed with FCM at 28 days after the last immunization. b , c Lung CD8 + and CD4 + T cells were evaluated for IFN-γ generation by FCM upon restimulation with RBD peptide pools or irrelevant antigens. b Representative FCM plots (left) and quantification (right) of IFN-γ-expressing lung CD8 + T cells. d The proportion of antigen-experienced (CD44 + ) CD4 + and CD8 + T cells expressing CD69 and CD103 in lung tissues. e The percentage of CD103 + DCs coexpressing CD86 and MHC II in the lungs. f Upper left: t-SNE maps were created by concentrating <t>CD3</t> + T cells from the BAL fluid of the vaccinated mice. Analysis was carried out with default FlowJo V.10 software. Upper right and bottom: heatmap projections of CD4, CD8, CD44, CD69, and CD103 expression on t-SNE maps from three independent mice ( n = 3). Red and black hashed circles are indicators of antigen-experienced CD4 + and CD8 + T RM cells in the BAL fluid, respectively. g Absolute numbers of CD8 + T cells producing IFN-γ (left) and TNF-α (right) in the BAL after ex vivo restimulation with RBD peptide pools or irrelevant antigen at 4 weeks after the last immunization. h Quantification of the percentages (left) and the numbers (right) of MHC II + AM in the BAL fluid. Data were displayed with floating bars in ( a – e , g , and h ). The middle line indicates the median and the box shows the data range. Data were presented as mean values ± SEM. n = 4–6 mice in each group in ( a – e , g , and h ). P values in a , d , e , h were calculated with One-way ANOVA followed by Dunnett’s multiple comparisons tests. P values in b , c , and g were calculated with two-way ANOVA followed by Tukey’s multiple comparisons tests. Source data are provided as a Source Data file.
Mouse Cd3 T Cell Column Enrichment Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd3 t cell isolation kit  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc cd3 t cell isolation kit
a The percentages of Tfh (CD4 + CXCR5 + PD-1 + ) and RBD-specific GCB (B220 + GL7 + CD95 + RBD + ) cells in the CLN were analyzed with FCM at 28 days after the last immunization. b , c Lung CD8 + and CD4 + T cells were evaluated for IFN-γ generation by FCM upon restimulation with RBD peptide pools or irrelevant antigens. b Representative FCM plots (left) and quantification (right) of IFN-γ-expressing lung CD8 + T cells. d The proportion of antigen-experienced (CD44 + ) CD4 + and CD8 + T cells expressing CD69 and CD103 in lung tissues. e The percentage of CD103 + DCs coexpressing CD86 and MHC II in the lungs. f Upper left: t-SNE maps were created by concentrating <t>CD3</t> + T cells from the BAL fluid of the vaccinated mice. Analysis was carried out with default FlowJo V.10 software. Upper right and bottom: heatmap projections of CD4, CD8, CD44, CD69, and CD103 expression on t-SNE maps from three independent mice ( n = 3). Red and black hashed circles are indicators of antigen-experienced CD4 + and CD8 + T RM cells in the BAL fluid, respectively. g Absolute numbers of CD8 + T cells producing IFN-γ (left) and TNF-α (right) in the BAL after ex vivo restimulation with RBD peptide pools or irrelevant antigen at 4 weeks after the last immunization. h Quantification of the percentages (left) and the numbers (right) of MHC II + AM in the BAL fluid. Data were displayed with floating bars in ( a – e , g , and h ). The middle line indicates the median and the box shows the data range. Data were presented as mean values ± SEM. n = 4–6 mice in each group in ( a – e , g , and h ). P values in a , d , e , h were calculated with One-way ANOVA followed by Dunnett’s multiple comparisons tests. P values in b , c , and g were calculated with two-way ANOVA followed by Tukey’s multiple comparisons tests. Source data are provided as a Source Data file.
Cd3 T Cell Isolation Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a The percentages of Tfh (CD4 + CXCR5 + PD-1 + ) and RBD-specific GCB (B220 + GL7 + CD95 + RBD + ) cells in the CLN were analyzed with FCM at 28 days after the last immunization. b , c Lung CD8 + and CD4 + T cells were evaluated for IFN-γ generation by FCM upon restimulation with RBD peptide pools or irrelevant antigens. b Representative FCM plots (left) and quantification (right) of IFN-γ-expressing lung CD8 + T cells. d The proportion of antigen-experienced (CD44 + ) CD4 + and CD8 + T cells expressing CD69 and CD103 in lung tissues. e The percentage of CD103 + DCs coexpressing CD86 and MHC II in the lungs. f Upper left: t-SNE maps were created by concentrating CD3 + T cells from the BAL fluid of the vaccinated mice. Analysis was carried out with default FlowJo V.10 software. Upper right and bottom: heatmap projections of CD4, CD8, CD44, CD69, and CD103 expression on t-SNE maps from three independent mice ( n = 3). Red and black hashed circles are indicators of antigen-experienced CD4 + and CD8 + T RM cells in the BAL fluid, respectively. g Absolute numbers of CD8 + T cells producing IFN-γ (left) and TNF-α (right) in the BAL after ex vivo restimulation with RBD peptide pools or irrelevant antigen at 4 weeks after the last immunization. h Quantification of the percentages (left) and the numbers (right) of MHC II + AM in the BAL fluid. Data were displayed with floating bars in ( a – e , g , and h ). The middle line indicates the median and the box shows the data range. Data were presented as mean values ± SEM. n = 4–6 mice in each group in ( a – e , g , and h ). P values in a , d , e , h were calculated with One-way ANOVA followed by Dunnett’s multiple comparisons tests. P values in b , c , and g were calculated with two-way ANOVA followed by Tukey’s multiple comparisons tests. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Cationic crosslinked carbon dots-adjuvanted intranasal vaccine induces protective immunity against Omicron-included SARS-CoV-2 variants

doi: 10.1038/s41467-023-38066-8

Figure Lengend Snippet: a The percentages of Tfh (CD4 + CXCR5 + PD-1 + ) and RBD-specific GCB (B220 + GL7 + CD95 + RBD + ) cells in the CLN were analyzed with FCM at 28 days after the last immunization. b , c Lung CD8 + and CD4 + T cells were evaluated for IFN-γ generation by FCM upon restimulation with RBD peptide pools or irrelevant antigens. b Representative FCM plots (left) and quantification (right) of IFN-γ-expressing lung CD8 + T cells. d The proportion of antigen-experienced (CD44 + ) CD4 + and CD8 + T cells expressing CD69 and CD103 in lung tissues. e The percentage of CD103 + DCs coexpressing CD86 and MHC II in the lungs. f Upper left: t-SNE maps were created by concentrating CD3 + T cells from the BAL fluid of the vaccinated mice. Analysis was carried out with default FlowJo V.10 software. Upper right and bottom: heatmap projections of CD4, CD8, CD44, CD69, and CD103 expression on t-SNE maps from three independent mice ( n = 3). Red and black hashed circles are indicators of antigen-experienced CD4 + and CD8 + T RM cells in the BAL fluid, respectively. g Absolute numbers of CD8 + T cells producing IFN-γ (left) and TNF-α (right) in the BAL after ex vivo restimulation with RBD peptide pools or irrelevant antigen at 4 weeks after the last immunization. h Quantification of the percentages (left) and the numbers (right) of MHC II + AM in the BAL fluid. Data were displayed with floating bars in ( a – e , g , and h ). The middle line indicates the median and the box shows the data range. Data were presented as mean values ± SEM. n = 4–6 mice in each group in ( a – e , g , and h ). P values in a , d , e , h were calculated with One-way ANOVA followed by Dunnett’s multiple comparisons tests. P values in b , c , and g were calculated with two-way ANOVA followed by Tukey’s multiple comparisons tests. Source data are provided as a Source Data file.

Article Snippet: T cells in untreated, female, 6–8 weeks BALB/c mouse spleen were obtained by a mouse CD3 T-cell enrichment kit (Stemcell).

Techniques: Expressing, Software, Ex Vivo